This second of three papers, in which we functionally characterize activation gating in Shaker potassium channels, focuses on the properties of a mutant channel (called V2), in which the leucine at position 382 (in the Shaker B sequence) is mutated to valine. The general properties of V2's ionic and gating currents are consistent with changes ...
WhatsApp: +86 18221755073This second of three papers, in which we functionally characterize activation gating in Shaker potassium channels, focuses on the properties of a mutant channel (called V2), in which the leucine at position 382 (in the Shaker B sequence) is mutated to valine. The general properties of V2's ionic and gating currents are consistent with changes in late gating …
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WhatsApp: +86 18221755073A Shaker Potassium Channel is a type of potassium channel that belongs to the Kv1 family, which includes Kv1, Kv2, and Kv3 potassium channels. AI generated definition based on: Reference …
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WhatsApp: +86 18221755073The potassium channels encoded by the Drosophila Shaker gene activate and inactivate rapidly when the membrane potential becomes more positive. Site-directed mutagene-sis and single …
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WhatsApp: +86 18221755073Calculations for the Modeling. For the modeling of Shaker's activation gating, we assume continuous-time, discrete-state Markov models, which have performed adequately at describing the gating processes of many other ion channels (for example, see McManus and Magleby, 1988).For all of the calculations, the numerical techniques described by Colquhoun …
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WhatsApp: +86 18221755073A Shaker Potassium Channel is a type of potassium channel that has been identified as a fundamental regulator of sleep behavior in organisms like fruit flies and mammals. In humans, auto-immunity to Shaker-type potassium channels can lead to conditions such as Morvan's syndrome, which may include symptoms like insomnia. ...
WhatsApp: +86 18221755073In this paper, we extend the kinetic analysis of the Shaker channel to the effects of the V2 mutation. This characterization of V2 serves two purposes. First, it serves to further …
WhatsApp: +86 18221755073K + channels of the Shaker family have been shown to play crucial roles in K + uptake and transport. Cotton (Gossypium hirsutum) is an important cash crop.In this study, the 24 Shaker family genes were identified in cotton. Phylogenetic analysis suggests that they were assigned to five clusters. Additionally, their chromosomal location, conserved motifs and gene …
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WhatsApp: +86 18221755073We calculated and compared the hydropathic profiles of two K (+) channels, KcsA and Kv1.2 (the latter a member of the Shaker family), at their pore-forming domain.
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WhatsApp: +86 18221755073The potassium channels encoded by the Drosophila Shaker gene activate and inactivate rapidly when the membrane potential becomes more positive. Site-directed …
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WhatsApp: +86 18221755073Voltage-activated potassium (Kv) channels open and close in response to changes in membrane voltage, serving critical functions in electrical signaling in neurons and muscle, neurotransmitter and hormone secretion, cell …
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WhatsApp: +86 18221755073For instance, Q-V curves of Shaker potassium channel obtained by hyperpolarizing from 0 mV is left-shifted compared to those obtained by depolarizing from a holding potential of −80 mV. This hysteresis in Q-V curves is a common feature of channels in the VGIC superfamily and raises profound questions about channel energetics because the net ...
WhatsApp: +86 18221755073We examined the effects of La 3+ on voltage-gated Shaker K + channels. Addition of the trivalent cation La 3+ (50 μM) extracellularly markedly prolongs the lag that precedes …
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WhatsApp: +86 18221755073The resting and activated conformations of the K V 1.2–2.1 voltage-sensing domain. (A) Experimentally determined activated conformation (Protein Data Bank ID code 2R9R).Note that R1 in Kv1.2–2.1 is glutamine, whereas R1 in Shaker is arginine. (B) Hypothetical resting/closed conformation.The yellow sphere represents the Ca 2+ in the hypothetical …
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WhatsApp: +86 18221755073For instance, Q-V curves of Shaker potassium channel obtained by hyperpolarizing from 0 mV is left-shifted compared to those obtained by depolarizing from a holding potential of −80 mV. This hysteresis in Q-V curves is a common feature of channels in the VGIC superfamily and raises …
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